毛竹BGLU家族成员鉴定及胁迫处理下的表达分析OA
Identification of BGLU Family Members in Moso Bamboo and Expression Analysis under Stress Treatment
[目的]β-葡萄糖苷酶(BGLU)是一种糖苷水解酶,参与植物的各种发育过程和胁迫反应.研究毛竹(Phyllostachys edulis)BGLU的分子特征与表达模式,对揭示其在毛竹速生及非生物胁迫应答中的作用具有重要意义.[方法]以毛竹为研究对象,利用生物信息学方法对其基因组中BGLU基因家族成员进行鉴定和系统分析,结合转录组数据筛选关键基因,构建共表达网络,利用qRT-PCR技术分析关键基因的表达模式,通过酵母单杂交(Y1H)和双荧光素酶报告(DLR)实验验证调控关系.[结果]在毛竹基因组中共鉴定出BGLU基因家族成员40个(PeBGLU1～PeBGLU40),不均匀分布在18条染色体上,有15对PeBGLUs呈现共线性.PeBGLUs的外显子数量为5～13个,编码蛋白序列长度为274～979 aa,均为亲水性蛋白.系统进化分析发现PeBGLUs分布于8个组中,其中Ⅶ组成员最多(13个),Ⅷ组无毛竹成员.PeBGLUs启动子序列中含有多种胁迫和激素响应元件,转录组数据分析发现叶片中PeBGLUs对干旱、盐胁迫和ABA具有不同程度的响应,其中PeBGLU25的表达变化最为显著,且与7个转录因子基因呈现共表达.qRT-PCR结果显示,Pe02464_MYB 与 PeBGLU25 表达趋势一致,Y1H 和 DLR 证实 Pe02464_MYB 能够与 PeBGLU25 启动子结合并激活其表达.[结论]毛竹中有40个BGLU基因家族成员,呈现不同的组织表达模式和非生物胁迫应答模式,其中PeBGLU25表达受Pe02464_MYB的转录调控,可能在毛竹应对干旱、盐胁迫和ABA响应中发挥着重要作用.
[Objective]β-glucosidase(BGLU)is a glycoside hydrolase involved in diverse plant develop-mental processes and stress responses.Elucidating the molecular characteristics and expression profiles of BGLU genes in moso bamboo(Phyllostachys edulis)is crucial for uncovering their functions in bamboo rapid growth and abiotic stress adaptation.[Methods]In this study,bioinformatics approaches were em-ployed to identify and systematically characterize the members of BGLU gene family in moso bamboo gen-ome.Transcriptome data were integrated to screen key genes,followed by construction of a co-expres-sion network.The expression patterns of key genes were analyzed using qRT-PCR technology,and the regulatory relationship was experimentally verified through yeast one-hybrid(Y1H)and dual luciferase re-porter(DLR)assays.[Results]A total of 40 members of BGLU gene family(designated as PeBGLU1 to PeBGLU40)were identified in moso bamboo genome,which distributed unevenly across 18 chromo-somes,with 15 pairs of PeBGLUs exhibiting collinearity.PeBGLUs contained 5 to 13 exons,and their en-coded proteins ranged from 274 to 979 amino acids in length,all of which were hydrophilic proteins.Phylo-genetic analysis revealed that PeBGLUs were divided into 8 groups,among which Group Ⅶ containing the largest number of members(13)and Group Ⅷ had no member of moso bamboo.The promoter se-quences of PeBGLUs harbored a variety of stress and hormone response elements.Transcriptome data analysis indicated that PeBGLUs in leaves responded to drought,salt stress,and abscisic acid(ABA)to different extents,among which the expression change of PeBGLU25 was the most significant and it was co-expressed with 7 transcription factor genes.qRT-PCR results confirmed that Pe02464_MYB and PeB-GLU25 exhibited similar expression trends.Further Y1H and DLR assays demonstrated that Pe02464_MYB could bind to the PeBGLU25 promoter and activate its expression.[Conclusion]In total,40 BGLU gene family members were identified in moso bamboo,and these genes exhibit distinct tissue-spe-cific expression patterns and abiotic stress response profiles.Notably,PeBGLU25 was transcriptionally regulated by Pe02464_MYB,suggesting that PeBGLU25 may play a critical role in the response of moso bamboo to drought,salt stress,and ABA treatment.
宛慧茹;李慧;朱成磊;寇单单;高志民
国际竹藤中心竹藤资源基因科学与基因产业化研究所竹藤科学与技术国家林业和草原局重点实验室国家以竹代塑创新研究院,北京 100102国际竹藤中心竹藤资源基因科学与基因产业化研究所竹藤科学与技术国家林业和草原局重点实验室国家以竹代塑创新研究院,北京 100102国际竹藤中心竹藤资源基因科学与基因产业化研究所竹藤科学与技术国家林业和草原局重点实验室国家以竹代塑创新研究院,北京 100102国际竹藤中心竹藤资源基因科学与基因产业化研究所竹藤科学与技术国家林业和草原局重点实验室国家以竹代塑创新研究院,北京 100102国际竹藤中心竹藤资源基因科学与基因产业化研究所竹藤科学与技术国家林业和草原局重点实验室国家以竹代塑创新研究院,北京 100102
农业科技
毛竹β-葡萄糖苷酶分子特征表达分析转录调控
Phyllostachys edulisbeta-glucosidasemolecular characterizationexpression analysistranscriptional regulation
《林业科学研究》 2026 (4)
34-46,13
国家重点研发计划项目课题2(2021YFD2200502)国家级
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