葛根素对过敏性哮喘小鼠疗效及肠道菌群调节作用OA

Objective This study aims to systematically evaluate the anti-asthma effect of puerarin,the main active ingredient in traditional medicinal plant Gegen(Puerariae Lobatae Radix),on a mouse model of allergic asthma,and explore whether it works by regulating the gut lung axis.Methods Using an ovalbumin-induced allergic asthma mouse model,mice were randomly divided into a control group(NC),an OVA model group(MO),an OVA+montelukast group(MK),an OVA+puerarin 20 mg/kg group(PL),and an OVA+puerarin 50 mg/kg group(PH).The effects of puerarin on asthma symptoms and related indicators were observed.To evaluate its efficacy by detecting pathological changes in the lungs,specific airway resistance,serum IgE,and eosinophil levels in bronchoalveolar lavage fluid;Simultaneously analyze the pathological and inflammatory cytokine changes in intestinal tissue.Using 16S rRNA sequencing technology to analyze the composition of gut microbiota,combined with Spearman correlation analysis to explore the relationship between microbiota and asthma severity,and conducting functional enrichment analysis through KEGG pathway annotation.Results Puerarin significantly reduced lung pathological damage in allergic asthma mice,decreased specific airway resistance,IgE,and eosinophil levels.Meanwhile,puerarin inhibits intestinal inflammation and pathological damage.The analysis of gut microbiota showed that puerarin significantly increased the abundance of Bacteroidetes(especially Porphyromonadaceae and Prevotellaceae)and decreased the abundance of Firmicutes(such as Ruminococcaceae,Lachnospiraceae,and Anaerobacterium).Correlation analysis shows that Anaerobacterium and unclassified_Lachnospiraceae are positively correlated with the severity of asthma,functional enrichment analysis suggests that changes in microbial communities are related to host metabolism and biosynthetic pathway regulation.Conclusion Puerarin has the potential to treat allergic asthma,and its mechanism of action may be related to regulating the structure of gut microbiota,especially increasing Bacteroidetes and reducing Firmicutes related bacteria,thereby affecting host immunity and metabolic pathways through the gut lung axis.