福建牡蛎(Crassostrea angulata)生长和粗蛋白含量性状的全基因组关联分析OA

The Fujian oyster(Crassostrea angulata)is an important shellfish species farmed in southern China;its growth rate and nutritional quality impact industry profitability.However,the genetic basis of key traits such as development and crude protein content remains poorly understood.To address this lacuna,we investigated 100 in-dividuals from a population cultured in Sanshan,Fuqing,Fujian Province,China,for six key traits:shell height,shell width,total weight,oyster weight,meat yield,and crude protein content.Whole-genome resequencing and genome-wide association studies were subsequently performed.All traits exhibited substantial phenotypic varia-tions(coefficient of variation:11.41%-53.21%).Growth traits were significantly positively correlated(P<0.05),but not with crude protein levels.Based on 15.9 million high-quality single-nucleotide polymorphisms(SNPs)and us-ing a mixed linear model,we detected 31 SNPs robustly associated with growth traits(-log10P≥6.0),distributed across chromosomes 1,2,5,7,9,and 10.Presumptive genes near these loci included pleiotropic genes(GCN2,FCN2,P2RX7,FER)and trait-specific genes(SMAD4 and TRIM2),primarily functioning in nutrient sensing,im-mune regulation,and growth.We also identified 15 SNPs enriched on chromosome 9 that were significantly associ-ated with crude protein content(-log10P≥9.2).In total,14 candidate genes located near these loci,including NCAN,HERC1,and SMARCA1,were involved in protein synthesis/degradation and epigenetic regulation.This study pre-liminarily screened SNPs and genes putatively associated with growth performance and crude protein contents in C.angulata.The findings provide targets and clues crucial for subsequent mechanistic genetic analyses and the identi-fication of molecular breeding markers.