多世代纳米镍暴露对海洋桡足类的综合生理影响与种群适合度研究OA

To assess the long-term ecological risks of nanometal pollutants to marine zooplankton,this study used the dominant copepod in the East China Sea,Acartia pacifica,as a model organism in multi-generational nano-nickel exposure experiments.The experiment set environmentally relevant concentration gradients(0.01 and 1.0 mg/L)and systematically measured key physiological and ecological parameters from the F0-exposed generation to the F2 recovery generation,including hatchability,survival rate,development times(including nauplius and gen-eration development times),female ratio,fecal pellets,oviposition,and growth rate(body length,body width).Nano-nickel significantly affected the physiology of Acartia pacifica in a dose-dependent and time-accumulative manner.Compared with the control,low concentration(0.01 mg/L)exposure did not significantly impact reproduc-tive parameters but significantly inhibited feeding capacity in the F0 and F1 generations(P<0.05),reflected by a decline in fecal pellets by 11.6%and 17.6%,respectively.Similarly,high concentration(1.0 mg/L)exposure com-prehensively suppressed growth and reproduction(P<0.05),reducing hatchability by 8.25%and 10.43%in the F0 and F1 generations,respectively.The survival rate of the F1 generation decreased by 12.02%,with feeding and re-productive capacities being co-inhibited.In the F2 generation,after exposure removal,although a few growth and reproductive indicators recovered,the feeding function did not fully recover in the high concentration group(P<0.05),with fecal production still significantly lower than the control by 1.69%.These results confirmed that nano-nickel can exert persistent multigenerational effects on key physiological functions in copepods,with residual impacts even after pollutant removal.This study reveals the potential long-term ecological risks of nano-nickel and provides experimental evidence crucial for improving the risk assessment of emerging pollutants in the marine en-vironment.