爽两优132双亲全基因组重测序分析及耐热相关基因验证OA

The national registered rice variety Shuangliangyou 132 is a two-line hybrid rice combination developed by the Hunan Hybrid Rice Research Center through crossbreeding between the sterile line Shuang 1S and the restorer line Shuanghui 32.This variety is characterized by high grain quality,strong heat tolerance,and high seed production yield.In this study,whole-genome resequencing was performed on Shuanghui 32 and Shuang 1S,with the japonica rice variety Nipponbare as the reference genome.The results indicated that 1.88～1.89 million SNP sites and 320 000～330 000 InDel sites were detected in both parental lines.Shuang 1S harbored 3 025 high-effect variants,while Shuanghui 32 had 3 108.Structural variant(SV)analysis revealed a highly consistent composition of SV types between Shuanghui 32 and Shuang 1S,with chromosomal rearrangements and fragment losses being the predominant forms,together accounting for over 80％of all SVs.Furthermore,first-generation sequencing validated the authenticity of variations in the heat tolerance genes GS3 and TTL1.These findings provide a significant foundation for mining functional genes in Shuang 1S and Shuanghui 32 and elucidating the molecular mechanisms underlying their heat tolerance,and offer data support for molecular-assisted breeding.