用于配体靶蛋白鉴定的溶剂诱导蛋白质沉淀方法的发展与应用OA

Drugs regulate biological processes by binding to target proteins.Characterizing drug and protein interactions is therefore critical for elucidating mechanisms of action,reducing toxic side ef-fects,overcoming drug resistance,and advancing novel drug discovery.Conventional target identifi-cation methods often require chemical modification of drugs,which tends to disturb native binding in-teractions and produce false-positive results.Solvent-induced protein precipitation(SIP)is a label-free approach that enables high-throughput drug target screening by detecting protein stabilization in-duced upon ligand binding,offering distinct advantages including simple operation and broad appli-cability.In recent years,researchers have developed a series of SIP-derived methods,including SPP,solvent-PISA,iSPP,and the live-cell in situ technique SICFA,which have greatly improved throughput and system compatibility.This review systematically summarizes the fundamental princi-ples,technological evolution,advantages,and limitations of SIP,and highlights its applications in target discovery and validation across cancer,Parkinson's disease,antimicrobial resistance,malar-ia,and other fields.We aim to provide a useful reference for precise target identification and innova-tive drug development.