饲用燕麦高效EMS诱变技术优化与高产种质筛选OA

In this study,the forage oat(Avena sativa L.)cultivar'Zhongtian No.4'was used as the experi-mental material to establish an optimized ethyl methanesulfonate(EMS)mutagenesis system.A series of pre-treatments steps were employed,including husk removal and a 30-hour seed moisture conditioning period,fol-lowed by EMS mutagenesis treatments at different concentrations and durations,and a post-treatment involving exogenous gibberellin application.An M2 mutant population was constructed.Agronomic traits were investi-gated at the milk stage to evaluate the mutagenic effects and to screen the high-yielding germplasm.The results indicated that soaking seeds in 98%concentrated sulfuric acid for 6 minutes effectively removed the husk barrier of forage oat seeds.Adjusting seed moisture content to 50%via a 4-hour soaking facilitated efficient penetra-tion of EMS into the seed.The optimal EMS mutagenesis conditions for forage oat were determined to be 1.0%EMS treatment for 16 hours.Treatment of mutagenized seeds with 50 mg·L-1 exogenous gibberellin sig-nificantly improved seedling growth.Compared with the control without added gibberellin,the germination rate increased by 8.5%,and the seedling length also recovered to the level of the non-mutagenized seedlings.This EMS mutagenesis technique systematically addressed key limiting factors in forage oat,including seed perme-ability barriers,inconsistent physiological states,and mutagen-induced damage.In the M2 generation,the mutagenic efficiency for obtaining high-yield potential germplasm reached 1.92%,and 13 elite high-yield potential germplasms were successfully identified.