乳酸菌发酵葛根菌株筛选及协同发酵工艺优化研究OA

Objective This study aimed to screen dominant lactic acid bacteria(LAB)for Gegen(Pueraria lobata)fermenta-tion and optimize the synergistic fermentation processes to enhance the bioactive compound yields.Methods By monitoring the dy-namic changes in bacterial activity,total acid,total flavonoids and antioxidant activity during the fermentation process of six strains of LAB,Analytic Hierarchy Process(AHP)-Entropy Weight Method was used to comprehensively evaluate and screen the domi-nant LAB and optimize the key process parameters such as strain ratio,inoculation amount and solid-liquid ratio based on the synergistic fermentation strategy of dominant bacteria.Results Lactobacillus plantarum P9 and Lactobacillus rhamnosus were the dominant strains for Gegen(Pueraria lobata)fermentation.The optimal process conditions for their synergistic fermentation were a strain ratio of 3∶1,inoculation volume of 10％,solid-liquid ratio of 1∶25,temperature of 37 ℃ and fermentation for 3 d.Un-der these conditions,the viable counts reached 8.96 lgCFU·mL-1(231.6％higher than Lactobacillus plantarum P9 monocul-ture,31.83％higher than Lactobacillus rhamnosus),total acid content peaked at 2.95 g·L-1(91.6％increase over Lactobacil-lus rhamnosus monoculture),and daidzein content achieved 1.32 mg·g-1(164％and 238％enhancements compared to respec-tive monocultures).Conclusion Microbial consortia integration synergistically amplified strain-specific advantages,significantly elevating bioactive metabolites and providing strain selection and process optimization strategy for the development of fermented functional beverages and probiotic preparations based on Gegen(Pueraria lobata).