清热逐风合剂对胶原诱导型关节炎大鼠 IL-17A、HIF-1α、VEGF、TGF-β表达的影响OA

Objective To observe the effects of Qingre Zhufeng Mixture on serum interleukin-17A(IL-17A),transforming growth factor-β(TGF-β),and the expression of hypoxia inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)in synovial tissue of rats with collagen-induced arthritis(CIA),a model of rheumatoid arthritis(RA),and to explore the mechanism by which Qingre Zhufeng Mixture alleviates the inflammatory response in RA.Methods A CIA rat model was established.Methotrexate tablets,tripterygium glycoside tablets,and Qingre Zhufeng Mixture were used as interventions.Paw swelling and arthritis index were measured.Hematoxylineosin(HE)staining was used to observe histopathological changes in synovial tissue.Serum levels of IL-17A and TGF-β were detected by enzymelinked immunosorbent assay(ELISA).The protein expression of HIF-1α and VEGF in synovial tissue was detected by Western Blot.Results Compared with the normal group,the model group showed significantly increased paw swelling and arthritis index(P＜0.01);significantly elevated serum IL-17A level and decreased serum TGF-β level(P＜0.01);and significantly increased protein expression of HIF-1α and VEGF in synovial tissue(P＜0.01).Compared with the model group,the Qingre Zhufeng Mixture group,methotrexate group,and tripterygium glycoside group all showed significantly reduced paw swelling and arthritis index(P＜0.01);significantly decreased serum IL-17A and increased serum TGF-β(P＜0.01);and significantly reduced protein expression of HIF-1α and VEGF in synovial tissue(P＜0.01).Conclusion Regulating the IL-17A/TGF-β balance to promote CD4+T cell differentiation toward regulatory T cells(Tregs),inhibiting the expression of HIF-1α and VEGF,and thereby restoring the Th17/Treg balance may be one of the molecular mechanisms by which Qingre Zhufeng Mixture alleviates the inflammatory response in rheumatoid arthritis.