姬松茸不同发酵方法栽培料的元素动态变化OA

Using fermented substrates of Agaricus blazei prepared by traditional manual fermentation and tunnel fermenta-tion as materials,three stages-unfermented mixed substrate,first fermentation,and second fermentation-were estab-lished.The physicochemical properties and elemental dynamic changes during the fermentation process were tracked and determined,aiming to provide a reference for optimizing green and efficient cultivation techniques of A.Blazei and inves-tigating the mechanism of cadmium enrichment.In accordance with agricultural industry standards,a total of 21 indica-tors were measured,including pH,moisture content,electrical conductivity,organic carbon,total nitrogen,total phospho-rus,total potassium,as well as Fe,Ca,Mg,Cu,Zn,Mn,La,Se,and 6 heavy metals(Ni,Pb,Cd,Cr,As,Hg).and multiple comparisons were conducted using Tukey's method.The results showed that the pH and C/N of the cultivation substrates from both fermentation methods decreased with the fermentation progress,while the content of various elements generally exhibited an upward trend.After the second fermentation,the tunnel fermentation yielded superior organic carbon content(36.13%),total nitrogen content(1.65%),and C/N(21.97)compared to the traditional manual fermentation(29.17%,1.17%,25.00).Regarding heavy metals,after the second fermentation in the traditional manual fermentation,the Pb con-tent reached 113.67 mg·kg-1,and the contents of Ni,Pb,Cd,Cr,and As were 1.63,15.49,2,2.96,and 2.24 times higher than those in the tunnel fermentation,respectively,significantly exceeding the limit values stipulated by relevant green food standards.In conclusion,during the fermentation process of A.Blazei cultivation substrates,the adoption of tunnel fermentation technology can produce substrates with better nutritional indicators and lower heavy metal content,effective-ly reducing the risk of heavy metal enrichment in fruiting body,and thereby laying a foundation for high-yield and high-quality cultivation of A.blazei.