皱木耳种质遗传特性评价及优良菌株筛选OA

To clarify the phenotypic traits and genetic differences of Auricularia delicata germplasm resource,screen excellent germplasm,and provide a reference for new variety breeding and germplasm innovation,this study used nine strains from different geographical origins as materials.Somatic incompatibility tests,ITS sequence identification,and SRAP molecular marker techniques were employed to analyze the genetic relationships among the strains,combined with a comprehensive evaluation based on the membership function of agronomic traits.The results showed that ITS sequence identification confirmed that all nine tested strains were A.delicata.In the somatic incompatibility tests,all 36 combina-tions exhibited obvious antagonistic lines,indicating differences among the strains.SRAP molecular marker cluster analysis revealed that at a similarity coefficient of 0.36,the tested strains could be divided into a southern group and a northern group,indicating differences among the strains.Significant differences were observed among strains in agronomic traits such as growth period,yield,ear color,and ear fold.According to the comprehensive membership function,strain C9 scored the highest(0.919),with excellent performance in growth period(77 d),yield(3.91 kg‧100 kg-1 dry substrate),and commercial traits.Strain C3 showed good commercial traits,with large ears and light yellow color.Strain C4 had a short growth period(77 d)and high yield(4.03 kg‧100 kg-1 dry substrate).Strain C5 featured reddish-brown ear color with vein-like folds on the ventral side.These four excellent germplasm resources can serve as parental materials for the selection and breeding of superior hybrid strains.