利用多组学技术研究菌株Pseudomonas sp.T4对上海青营养品质的影响OA

In this study,a strain of Pseudomonas sp.T4,isolated from the root of Shanghaiqing,was used as the experi-mental material.Pot experiments were conducted to evaluate the effects of strain T4 on biomass accumulation,as well as the content of chlorophyll,protein,soluble sugar,mineral element,and the metabolic finger printing of Shanghaiqing.The results showed that inoculation with T4 significantly increased the biomass of Shanghaiqing,where the fresh mass of root and shoot were 1.58 and 1.17 times higher than those of the non-inoculated control,respectively.Physiological index anal-ysis revealed that the content of chlorophyll,protein,and soluble sugar in the leaves of T4-inoculated Shanghaiqing were significantly increased by 19.49%,10.88%,and 69.12%compared to the control,respectively.Non-targeted metabolo-mics analysis indicated that,compared with the control,T4 inoculation significantly increased the levels of vitamins(nic-otinamide,4-pyridoxic acid,and pyridoxine),flavonoids(cyanidin-3-O-glucoside,luteolin-4′-O-glucoside and querce-tin-3,4′-diglucoside),and fatty acids(palmitoleic acid,oleic acid,and linolenic acid)in the shoots.Ionomics analysis demonstrated that the content of mineral elements including K,Mg,Cu,Ba,and B in T4-treated Shanghaiqing were signif-icantly increased,ranging from 1.06 to 1.48 times those of the control.In conclusion,strain T4 not only significantly en-hances the biomass but also markedly improves the nutritional quality of Shanghaiqing.This study provides microbial re-sources and a theoretical basis for the development of microbial agents.