不同栽培料培养下毛木耳转录组分析OA

To explore the effects of different cultivation substrates on the growth,development and metabolic mechanism of Auricularia cornea,this study used a control group(CK)with cottonseed hulls as the main substrate(84%cottonseed hulls,13%bran,and 3%gypsum)and a treatment group(T1)with a composite substrate of corncob,sawdust,and cotton-seed hulls(28%corncob,28%sawdust,28%cottonseed hulls,13%bran,and 3%gypsum).Fruit bodies of A.cornea were cultivated separately under these two conditions,and transcriptome sequencing of the fruiting bodies was performed using the Illumina HiSeq sequencing platform.The results showed that a total of 291.87 M clean reads were obtained from the two groups,with Q20 base quality scores above 95%in all samples.Differentially expressed genes(DEGs)analy-sis revealed that compared with the control group,a total of 4847 DEGs were identified in the treatment group,including 141 up-regulated genes and 4706 down-regulated genes.GO functional enrichment analysis revealed that the DEGs were mainly enriched in biological processes such as translation and peptide metabolism processes.KEGG pathway enrichment analysis indicated that the DEGs were significantly enriched in pathways related to metabolism and protein synthesis,in-cluding ribosome,oxidative phosphorylation,phagosome,and protein processing.Quantitative real-time PCR(qRT-PCR)validation results were consistent with the transcriptomic data,confirming the reliability of the sequencing results.This study provides a molecular basis for understanding the adaptive metabolic mechanism of A.cornea to composite cultiva-tion substrates,and also offers theoretical support for optimizing the substrate formula for A.corneacultivation.