丁酸钠和藤茶黄酮对澳加F1代荷斯坦断奶母犊牛瘤胃发酵参数和微生物区系的影响OA

The purpose of this experiment was to investigate the effects of sodium butyrate and ampelopsis grossedentata flavonoids on rumen fermentation parameters and microflora of weaned female calves.Twenty-eight healthy Holstein weaned female calves of similar age[(83.39±6.02)d]and body weight[(98.82±3.23)kg]were randomly divided into 4 groups,and 7 test cattle per group.Group Ⅰ was the control group,and calves in the group Ⅰ were fed a basal diet.Groups Ⅱ,Ⅲ and Ⅳ were test groups,and sodium butyrate at 3 g/kg DM,ampelopsis grossedentata flavonoids at 8 g/kg DM,and a combination of sodium butyrate at 3 g/kg DM and ampelopsis grossedentata flavonoids at 8 g/kg DM were respectively added to the control group.The results showed as follows:The contents of acetic acid,butyric acid and isovaleric acid in rumen fluid of group Ⅳ was significantly lower than that of group Ⅰ(P<0.05),the content of valeric acid in rumen fluid of group Ⅲ and group Ⅳ was significantly lower than that of group I(P<0.05).The rumen microbialflora Chao1 index of group Ⅲ was significantly higher than that of group I(P<0.05).At the phylum level,the relative abundance of Bacteroidetes in rumen of groups Ⅲ and Ⅳ was significantly lower than that of groups Ⅰ and Ⅱ(P<0.01).The relative abundance of Firmicutes was significantly higher than that of groups Ⅰ and Ⅱ(P<0.05);the relative abundance of Proteobacteria in rumen of group Ⅲ was significantly higher than that in group Ⅰ and group Ⅳ(P<0.01);the relative abundance of Patescibacteria in rumen of group Ⅳ was significantly higher than that of group Ⅰ(P<0.05),and the relative abundance of Tenericutes was significantly higher than that of group Ⅰ and II(P<0.05).At the genus level,the relative abundances of Prevotella_1 and Muribaculaceae in rumen of group Ⅳ were significantly higher than those of group Ⅰ and group Ⅱ(P<0.05);the relative abundances of Christensenellaceae_R-7_group and Ruminococcaceae_NK4A214_group in rumen of groups Ⅲ and Ⅳ were significantly higher than those of group Ⅰ and group Ⅱ(P<0.05);The relative abundances of Rikenellaceae_RC9_gut_group and F082 in rumen of group Ⅲ and groupⅣ were significantly higher than those of group I(P<0.05);The relative abundances of Ruminococcaceae_UCG-014 in rumen of group Ⅲ were significantly higher than those of group Ⅰ and group Ⅱ(P<0.05);the relative abundance of Prevotellaceae NK3B31_group in rumen of group Ⅱ was significantly higher than that of the other three groups(P<0.05);and the relative abundances of Ruminococcus_1 and Butyrivibrio_2 in rumen of group Ⅳ were significantly higher than those of group I(P<0.05);The relative abundance of Lachnospiraceae_XPB1014_group in rumen of groups Ⅱ and Ⅲ was significantly higher than that of group Ⅰ(P<0.05).In conclusion,the addition of SB alone was more conducive to rumen fermentation and improve the energy utilization efficiency of calves.Adding AGF alone could significantly increase the rumen flora richness and the relative abundance of beneficial bacteria of calves.The simultaneous addition of SB and AGF had no synergistic effect on rumen fermentation,proliferation of beneficial bacteria and metabolic regulation.