储存时间对茅苍术挥发性成分分析及其抗炎活性的影响OA

The study aimed to investigate the impact of storage duration on the quality of Atractylodes lancea(AL).GC-MS was employed to systematically analyze the dynamic changes in volatile components of AL stored for different periods(0,1year,and 2 years),while LPS-induced RAW 264.7 murine monocyte-macrophage inflammatory models were utilized to evaluate anti-inflammatory activity.The results demonstrated that characteristic volatile components,including atractylodin,hinesol,and β-eudesmol,exhibited the highest initial content during fresh storage(0 year,accounting for 40.71%,19.37%and 6.39%of total volatile components,respectively),with a gradual decline observed over extended storage periods.Cellular experiments revealed no cytotoxicity for volatile components across all storage groups at 50 μg/mL(cell viability>90%),with consistent inhibition of LPS-induced NO release(inhibition rate:29.08%～31.75%).Notably,the 1-year-stored AL displayed a relatively optimal anti-inflammatory trend.This study elucidate the nonlinear correlation between the storage stability of volatile components in AL and their anti-inflammatory efficacy,providing critical experimental evidence for establishing scientific storage standards of traditional Chinese medicinal materials.