响应面优化黑果腺肋花楸总三萜的提取及其抑菌活性和稳定性研究OA

The experiment used Aronia melanocarpa as the raw material and employed ultrasonic-assisted ethanol extraction to extract total triterpenes.The extraction process was optimized using response surface methodology(RSM)with the goal of maximizing the total triterpene content in Aronia melanocarpa.The antibacterial activity,antioxidant capacity,hemolytic activity,cytotoxicity,and stability of the extract were systematically evaluated.The results indicated that the optimal extraction conditions for total triterpenes were an alcohol concentration of 61%,a solid-to-liquid ratio of 1:36(g/mL),and an extraction time of 80 min,yielding a total triterpene content of 4.442 mg/g.The extract demonstrated significant antimicrobial activity against Escherichia coli and Staphylococcus aureus,with minimum bactericidal concentration(MBC)of 8 mg/mL and 1.25 mg/mL,respectively.Additionally,at a concentration of 0.25 mg/mL,the extract exhibited a free radical scavenging rate of up to 99%,showing excellent antioxidant capacity.Furthermore,the hemolysis rate of the extract was below 10%at concentrations 10～1.25 mg/mL,and cytotoxicity tests revealed over 95%cell viability after 12 hours,indicating good biosafety and cell selectivity.However,the extract showed some sensitivity in environments containing salt ions and enzyme solutions,with the MBC increasing 2～8 times.This research provides a theoretical basis for ennancing the deep-processing level and the added value of Aronia melanocarpa products,as well as its applications in the fields of food additives and animal feed additive.