白头翁提取物对肉羊生长性能、血清抗氧化指标及瘤胃发酵参数的影响OA

This study investigated the effects of different supplemental levels of Pulsatilla extract(PE)on growth performance,serum antioxidant indices and rumen fermentation parameters of mutton sheep,and to determine the optimal addition amount of PE in the diet of mutton sheep.A total of 180 Hu sheep male lambs with an initial body weight of approximately 25 kg were divided into 5 groups.CK was fed a basal diet,and the four experimental groups(PE1,PE2,PE3 and PE4)were fed the basal diet supplemented with 200,400,800 and 1600 mg/kg Pulsatilla extract,respectively.The results showed that:1)PE1 showed no significant difference from the CK(P＞0.05).Compared with CK,FW of PE2 and PE3 were significantly enhanced by 5.84%and 8.96%(P＜0.05),ADG by 8.69%and 13.37%(P＜0.05),and F/G were significantly reduced by 6.67%and 9.33%(P＜0.05),respectively,with PE3 showing significantly better effects than PE2(P＜0.05).In contrast,FW and ADG of PE4 were significantly reduced by 6.12%and 17.01%compared with CK(P＜0.05),while F/G was significantly increased by 17.00%(P＜0.05).2)Regarding serum antioxidant indices,the total antioxidant capacity,inhibition of hydroxyl free radical capacity,glutathione peroxidase,catalase and superoxide dismutase activities in PE2 and PE3 were significantly enhanced(P＜0.05),while MDA was significantly lower than that in CK(P＜0.05),and PE3 showed significantly better results than PE2(P＜0.05).No significant differences were observed in ALT,AST,BUN or CREA among CK,PE1,PE2 and PE3 groups(P＞0.05).All antioxidant indices in PE4 were significantly inferior to those of CK(P＜0.05),while ALT,AST,BUN and CREA in PE4 were significantly higher than those in CK(P＜0.05).3)Regarding rumen fermentation parameters,the microbial protein and total volatile fatty acid of PE2 and PE3 were significantly higher than those in CK(P＜0.05),while the ammonia nitrogen content was significantly lower than that in CK(P＜0.05),and PE3 showed significantly better results than the PE2(P＜0.05).All rumen fermentation parameters in PE4 were significantly inferior to those of CK(P＜0.05).Based on comprehensive evaluation of all indices,the optimal addition amount of PE is 400-800 mg/kg,with the 800 mg/kg addition showing the best improvement.An addition amount exceeding 1600 mg/kg exerts significant adverse effects on mutton sheep.