丁酸梭菌对郏县红牛犊牛生长性能、腹泻率、血清免疫和抗氧化指标、粪便短链脂肪酸浓度的影响OA

This study aimed to investigate the effects of dietary Clostridium butyricum supplementation on the growth performance,immune function,antioxidant capacity,and fecal short-chain fatty acid(SCFA)profile of Jiaxian Red cattle calves,providing a theoretical reference for its scientific application in the healthy breeding of this breed.A total of 48 healthy Jiaxian Red cattle calves[(1-day-old,(40.56±2.23)kg,equal number of males and females]were randomly divided into 4 groups,with 3 replicates per group and 4 calves per replicate.The control group was fed a basal diet,while the diets of GroupsⅠ,Ⅱ,and Ⅲ were supplemented with 1,3,and 5 g/(head·d)of C.butyricum,respectively.The experiment lasted for 60 days.The results showed that compared with the control group,the diarrhea incidence in trial groups Ⅰ,Ⅱ,and Ⅲ was significantly reduced by 37.96%,44.75%,and 41.67%,respectively(P＜0.05).Serum IgM levels in groups Ⅰ and Ⅲ were significantly increased by 5.76%and 8.56%(P＜0.05),respectively.The IL-2 content in group Ⅱ was significantly elevated by 12.24%(P＜0.05).TNF-α concentrations in groups Ⅱ and Ⅲ were significantly decreased by 16.14%and 11.43%(P＜0.05),respectively.MDA levels in groups Ⅱ and Ⅲ were significantly lowered by 8.33%and 16.03%(P＜0.05),while T-AOC activity in group Ⅲ was significantly enhanced by 9.95%(P＜0.05).Fecal propionic acid in groups Ⅱ and Ⅲ increased significantly by 20.78%and 26.54%(P＜0.05),and fecal butyric acid rose markedly by 16.79%and 21.37%(P＜0.05),respectively.In conclusion,the addition of Clostridium butyricum to the diet enhances the immune function and antioxidant capacity of Jiaxian Red cattle calves,regulates the production of short-chain fatty acids in the intestines,and reduces the diarrhea rate.Under the experimental conditions,an addition level of 3 g per head per day demonstrated optimal efficacy.