基于多分子标记的唐松草属发育与物种鉴定OA

DNA barcoding technology has emerged as a significant tool for species identification and has shown re-markable advantages in the fields of plant taxonomy and traditional Chinese medicine identification in recent years.This study takes six species of the genus Thalictrum as the research objects,combines four molecular markers-ITS,rbcL,matK,and ycf1-and investigates their phylogenetic relationships and the accuracy of species identification through PCR amplification,Sanger sequencing,BLAST alignment,and phylogenetic analysis.The results show that all samples were successfully amplified with the target sequences,among which the ITS and ycf1 markers performed optimally in species differentiation,with a support rate of up to 100%.The phylogenetic tree indicates that all six samples belong to the subgenus Thalictrum(Thalictrum subg.Thalictrum),where T1 was determined to be Thal-ictrum baicalense using molecular methods,and T5 is highly related to Thalictrum petaloideum.This study verifies the reliability of the combined application of multiple molecular markers and provides molecular evidence for the tax-onomic revision of Thalictrum plants and the identification of traditional Chinese medicinal materials.