白花败酱中2个新的无环单萜类成分OA

Objective To investigate the chemical constituents of the n-butanol fraction of Patrinia villosa.Methods The compounds were isolated and purified by various chromatographic techniques including silica gel,ODS,Sephadex LH-20 gel,and semi-preparative HPLC,and their structures were elucidated by analysis of spectroscopic data.The anti-inflammatory activities were evaluated by against NO production from lipopolysaccharide(LPS)-stimulated RAW264.7 cells.Results A total of 14 compounds were isolated from the n-butanol fraction of P.villosa ethanol extract and identified as(2E,5R,6R)-5,8-dihydroxy-2,6-dimethyl-2-octenoic acid(1),(2E,5R,6R)-8-acetoxy-5-hydroxy-2,6-dimethyl-2-octenoic acid(2),(2E,6R)-8-hydroxy-2,6-dimethyl-2-octenoic acid(3),(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one(4),ethyl chlorogenate(5),ethyl caffeate(6),dihydrosyringenin(7),7S,8S-threo-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan(8),(+)-isolariciresinol(9),(7S,8R)-dihydrodehydrodiconiferyl alcohol(10),ceplignan(11),(+)-syringaresinol(12),salicifoliol(13),and 5-hydroxy-4-(4-hydroxyphenyl)-2(5H)-furanone hydroxy-butenolide(14).Compounds 1 and 2 are new compounds,and their NO inhibition rates at a concentration of 50 μmol/L were(6.16±1.41)％and(11.11±2.10)％,respectively.Conclusion Compounds 1 and 2 are new acyclic monoterpenoids,and namely patrivic acid B and 8-acetylpatrivic acid B.Compounds 3,4,10,11 and 14 were isolated from P.villosa for the first time.Compounds 1 and 2 showed no significant anti-inflammatory activity.