氧化磷酸化相关基因在骨关节炎免疫微环境中的生物信息学分析OA

Objective To investigate the role of oxidative phosphorylation-related genes in the pathogenesis of osteoarthritis and their relationship with the immune microenvironment.Methods Based on osteoarthritis synovial tissue datasets(GSE55235 and GSE55457)from Gene Expression Omnibus(GEO)database,differentially expressed genes related to oxidative phosphorylation were screened through differential expression analysis,and their mechanisms were explored using functional enrichment(GO/KEGG),immune infiltration(ssGSEA/CIBERSORT),and regulatory network(PPI,miRNA-TF-RBP)analysis.Results Fifteen differentially expressed genes related to oxidative phosphorylation were identified,which were significantly enriched in pathways such as mitochondrial respiratory chain complex assembly and oxidative phosphorylation(P<0.05).Immune infiltration analysis revealed significantly elevated levels of 16 immune cell types in the osteoarthritis group(P<0.05);correlation analysis indicated that TWF2 was positively correlated with macrophage infiltration(r=0.69),while UCP2 was negatively correlated with resting CD4 memory T cells(r=-0.67).The regulatory network revealed interactions among 8 core oxidative phosphorylation genes,which were modulated by 25 miRNAs,27 TFs,and 20 RBPs.Conclusion Oxidative phosphorylation-related genes(TWF2,UCP2)drive the pathological progression of osteoarthritis by regulating macrophage polarization and T cell metabolic reprogramming,thereby inducing immune microenvironment imbalance.