内障丸对早发性卵巢功能不全的作用效果分析OA

Objective:To explore the effect of Neizhang Pill on Premature Ovarian Insufficiency(POI).Methods:Forty SPF grade healthy female SD rats were subcutaneously injected with 200 mg/kg D-galactose in the head neck triangle area for 42 consecutive days to establish a premature ovarian insufficiency(POI)model.The SD rats were randomly divided into a blank control group(NC),a model control group(MC),a positive control group(PC),a low-dose Neizhang Pill group(L),a medium dose Neizhang Pill group(M),and a high-dose Neizhang Pill group(H)according to body weight.HE staining was used to observe the pathological status of rat ovarian tissue,immunohistochemistry was used to observe the expression levels of estrogen receptor(ER)and progesterone receptor(PR)in rat ovarian tis-sue,and ELISA was used to observe the contents of estradiol(E2),follicle stimulating hormone(FSH),luteinizing hormone(LH),and interleukin-6(IL-6).Results:HE results showed that Neizhang Pill can increase the number of mature follicles,reduce the number of blocked follicles,increase the volume of corpus luteum,and normalize the mor-phology of granulosa cells around follicles in a rat model of early-onset ovarian dysfunction.The immunohistochemical results showed that treatment with Neizhang Pill can increase the expression levels of ER and PR in the ovaries of a rat model of early-onset ovarian dysfunction.The ELISA method showed that Neizhang Pill can reduce the levels of FSH,LH,and IL-6 in the ovaries of rats with early-onset ovarian dysfunction,and increase the level of E2.Conclusion:The intervention effect of Neizhang Pill on POI is significant,and it can effectively promote the recovery of the reproductive system level in a rat model of early-onset ovarian dysfunction,providing support for clinical promotion.