基于代谢组学探讨电针预处理对脑缺血损伤大鼠脑皮质代谢物的影响OA

Objective:To utilize non-targeted metabolomics techniques so as to investigate the effects of electroacupunc-ture pretreatment on the metabolite profile of the cerebral cortex in rats with ischemic brain injury,and to clarify the molecular pathways of the neuroprotective effect of electroacupuncture pretreatment.Methods:For the experimental design,thirty male Sprague-Dawley rats were allocated into three groups using a randomization process:sham operation group,model group,and electroacupuncture preconditioning group(n=10 per group).In the electroacupuncture pre-conditioning group,rats underwent electroacupuncture stimulation at acupoints"Baihui(GV20)",bilateral"Neiguan(PC06)",and"Sanyinjiao(SP6)".The stimulation parameters consisted of dense-disperse waveforms at a frequency of 2/15 Hz,administered for 20 minutes per session over five consecutive days.Following the final electroacupuncture ses-sion,two hours later,the middle cerebral artery occlusion model was induced via intraluminal suture embolization.The sham operation group and model group were only subjected to grasping and binding without electroacupuncture inter-vention.At 24h after modeling,neurological function was evaluated by Garcia JH score,pathological changes of brain tissue were observed by hematoxylin-eosin(HE)staining,and changes in cerebral cortical metabolites were analyzed by non-targeted metabolomics technology.Results:When compared to the sham operation group,the model group ex-hibited a significantly reduced Garcia JH score(P<0.05).Histopathological analysis via HE staining revealed disor-ganized brain tissue with marked edema in the ischemic penumbra cortex.In contrast,the electroacupuncture precondi-tioning group demonstrated a statistically elevated Garcia JH score compared to the model group(P<0.05),with HE staining showing well-defined cortical layers and decreased edema in the ischemic penumbra region.Metabolomic profi-ling identified 16 differentially expressed metabolites between the model and electroacupuncture preconditioning groups,including 8 up-regulated and 8 down-regulated metabolites.Pathway analysis showed that electroacupuncture preconditioning exerted brain protection mainly by regulating riboflavin metabolism and glutathione metabolism path-ways.Conclusion:Electroacupuncture preconditioning can alleviate neurological deficit and improve pathological dam-age of brain tissue in rats with cerebral ischemic injury,and its brain protective mechanism is related to regulating ribo-flavin metabolism,glutathione metabolism,and other pathways.