千层纸素A对胃癌SGC-7901细胞的影响及其调控铁死亡机制研究OA

The study investigated the effects and mechanisms of oroxylin A on gastric cancer SGC-7901 cells.The methods employed included cell viability assay kit,Hoechst 33258 fluorescent probe,cell scratch assay,Transwell assays,reactive oxygen species(ROS)fluorescent probe,JC-1 fluorescent probe,ferrous ion(Fe2+)fluorescent probe,network pharmacology,and Western blot to explore the impact of oroxylin A on cell viability,apoptosis,migration,invasion,and ferroptosis,as well as its regulatory mechanisms of ferroptosis in SGC-7901 cells.The results revealed that oroxylin A inhibited SGC-7901 cell viability,scratch healing,migration,and invasion,as well as induced apoptosis,ROS accumulation,mitochondrial membrane potential depolarization,and Fe2+levels.Additionally,oroxylin A reduced the expression of the epidermal growth factor receptor(EGFR)protein in cells.The EGFR agonist NSC 228155 can reverse the increase in Fe2+levels and the decrease in cell viability induced by oroxylin A.The results indicate that oroxylin A exhibits a clear anticancer effect on gastric cancer SGC-7901 cells and induces cellular ferroptosis through the inhibition of EGFR.