响应面优化黄精多糖超声辅助复合酶提取工艺与活性分析OA

To promote the deeper exploitation of Polygonatum sibiricum polysaccharides in pharmaceutical and food fields,the ultrasound-assis-ted complex enzyme extraction of polysaccharides from Polygonatum sibiricum sourced from Fanjing Mountain was optimized via response surface methodology,followed by an assessment of its biological activity.The results indicated that the optimal extraction conditions were as follows:com-plex enzyme concentration of 6%,ultrasound-assisted enzymolysis time of 37 min,and ethanol precipitation time of 46 min.Under the condi-tions,the polysaccharides content reached 47.72%and the polysaccharides demonstrated good antioxidant activity with DPPH radical scavenging rates of 54.18%and ABTS radical scavenging rates of 32.77%at a concentration of 8 mg/mL.