基于细胞的卷烟烟气中主要甜感物质的甜度值测定OA

To overcome the limitations of current sweetness measurement methods for cigarette smoke and achieve objective quantification of sweet sensory sensation,HEK293 cell lines with the overexpression of human sweet taste receptor T1R2/T1R3 were established.By employing calcium ion imaging technology to monitor real-time changes in intracellular calcium signals induced by sweet substances,quantitative characterization of sweetness response was realized.Using this technique,sweetness values of ten previously reported representative sweet substances in cigarette smoke including furfural and furanone were systematically determined.Furthermore,the influence of the total particulate matter(TPM)on the sweetness performance of these sweet substances was investigated.The results showed that:1)Most sweet substances exhibited varying degrees of reduced sweetness values in TPM matrix,which may be attributed to the competitive or non-competitive inhibition on the sweet receptors by other complex smoke components.2)Partial substances(fenugreek lactone and 3,5-dimethyl-1,2-cyclopentanedione)maintained stable or slightly enhanced sweetness in the TPM matrix,suggesting that the smoke matrix exerted differential effects on sweetness of different sweet substances and might even involve synergistic effects.These findings suggest that cigarette flavor regulation should focus on components that exhibit stable or enhanced sweetness in the smoke matrix.