苏木乙酸乙酯提取物调控PI3K/Akt信号通路对冠脉血管新生的影响OA

Objective It is to observe the effects of sappanwood ethyl acetate extract on coronary angiogenesis and PI3K/Akt signal pathway in rats with acute myocardial infarction,and to explore its mechanism of action in alleviating myo-cardial injury.Methods Eighty male SD rats were randomly divided into blank group,sham operation group,model group and sappanwood group,with 20 rats in each group.The rats of the control group were normally fed,the rats of the sham op-eration group underwent thoracotomy and threading without ligation of left anterior descending coronary artery,the rats of the model group and sappanwood group underwent ligation of left anterior descending coronary artery to establish acute myo-cardial infarction models.After recovery from anesthesia,the sappanwood group was administrated 50 mg/kg of sappanwood ethyl acetate extract via gavage,while the other groups received an equal volume of distilled water via gavage,all adminis-tered once daily,continuously treated for 14 days.On the 1st,7th and 14th days after surgery,the percentage of myocardi-al infarction area in rats of each surgical group was detected by TTC staining;after the final gastric administration,the pathological morphology of myocardial tissue and the microvascular density in the infarct margin were observed by HE stai-ning,the Matrigel plug angiogenesis index was calculated by flow cytometry,the mRNA expressions of PI3K,Akt,and VEGF in myocardial tissue were detected by RT-qPCR,and the protein expressions of p-PI3K,PI3K,p-Akt,Akt,and vascular endothelial growth factor(VEGF)in myocardial tissue were detected by Western blot.Results On the 1st,7th and 14th days after surgery,the percentages of myocardial infarction area in the model group and sappanwood group were significantly higher than those in the sham operation group at the same period(all P<0.05);on the 7th and 14th days af-ter surgery,the percentages of myocardial infarction area in the sappanwood group were significantly lower than those in the model group at the same period(all P<0.05).Compared with the sham operation group,large areas of necrosis and rup-tured myocardial fibers in myocardial tissue were found in rats of the model group;the microvascular density in infarct mar-gin and the Matrigel plug angiogenesis index were significantly increased(all P<0.05);the relative mRNA expressions of PI3K,Akt and VEGF,as well as the relative protein expressions of p-PI3K/PI3K,p-Akt/Akt and VEGF in myocardial tis-sue were all significantly reduced(all P<0.05).Compared with model group,the area of myocardial necrosis was obvi-ously decreased with increased number of microvessels in rats of the sappanwood group;the microvascular density in infarct margin and the Matrigel plug angiogenesis index were significantly increased(all P<0.05);the relative mRNA expres-sions of PI3K,Akt and VEGF,as well as the relative protein expressions of p-PI3K/PI3K,p-Akt/Akt and VEGF in myo-cardial tissue were all significantly increased(all P<0.05).Conclusion Sappanwood ethyl acetate extract can alleviate myocardial injury,increase microvessel density in infarct margin,and enhance the Matrigel plug angiogenic response in rats with acute myocardial infarction.These effects may be associated with the activation of the PI3K/Akt signaling pathway and the upregulation of VEGF expression.