保卵安坤方对卵巢储备功能减退小鼠生育力的影响OA

Objective It is to evaluate the effect of Bao Luan An Kun Formula(BLAKF)on fertility in mice with di-minished ovarian reserve(DOR).Methods A total of 74 ICR female mice with normal estrous cycles were taken and ran-domly divided into blank group(n=22),model group(n=22),Chinese medicine group(n=10),western medicine group Ⅰ(n=10),and western medicine group Ⅲ(n=10).The mice of all groups except for the blank group were trea-ted with cisplatin via intraperitoneal injection for 7 days to establish DOR models.From the start of modeling,the Chinese medicine group was administrated 0.3 mL of BLAKF solution(0.52 g/mL)via gavage,while the blank and model groups were administrated an equal volume of distilled water via gavage,all continuously treated for 14 days.The western medicine group Ⅰ was treated with 0.3 mL of distilled water via gavage for 10 days,0.3 mL of clomiphene citrate solution(0.76 mg/mL)for 2 days,and 0.3 mL of distilled water for 2 days;the western medicine group Ⅲ was treated with 0.3 mL of clomiphene citrate solution(0.76 mg/mL)via gavage for 2 days,0.3 mL of distilled water for 3 days,0.3 mL of clomiphene citrate so-lution(0.76 mg/mL)for 2 days,0.3 mL of distilled water for 3 days,0.3 mL of clomiphene citrate solution(0.76 mg/mL)for 2 days,and 0.3 mL of distilled water for 2 days.After 14 days of gavage,10 female mice from each group were paired with males at a 2∶1 ratio for mating,and the specimens were collected on the 14th gestational day.The remaining 12 fe-male mice in the blank and model groups were not paired and were sampled at the beginning and end of the mating period.The general condition,body weight,and estrous cycle change of mice in all groups were observed.The ovaries of non-ma-ted mice were weighted and the ovarian organ index was calculated,the serum levels of anti-Müllerian hormone(AMH),follicle-stimulating hormone(FSH)and estradiol(E2)were measured.The numbers of pregnant,infertile,and pseudo-pregnant mice from the mated mice were recorded,and the implantation rate,embryo survival rate,pre-implantation loss rate,and post-implantation loss rate of the pregnant mice were calculated,the embryonated uterus of pregnant mice was weighted,and the organ index of embryonated uterus was calculated,the surviving embryos and placenta were weighted,the body length of surviving embryos and diameter of placenta,the serum levels of estradiol(E2),progesterone(P)and human chorionic gonadotropin(HCG)in pregnant mice were detected.Results The mice in the model group showed re-duced food intake,increased water intake,dry,coarse and unruly hair,decreased activity,weight loss,and prolonged es-trous cycles;these conditions were significantly improved in the Chinese medicine group,with a tendency of weight increase compared with the model group and no obvious prolongation of estrous cycles;these conditions were not improved in the mice of the western medicine groups Ⅰ and Ⅲ,with more weight loss and severe disruption of estrous cycles compared with the model group.At the beginning and end of the mating period,the ovarian weight,ovarian organ index,and serum levels of AMH and E2 of female mice in the model group were significantly lower than those in the blank group(all P<0.05),while serum level of FSH was significantly higher than that in the blank group(P<0.05).No significant difference was found in the number of successfully pregnant mice between the model group and blank group(P>0.05);the number of successfully pregnant mice in the western medicine group Ⅲ was significantly lower than that in the blank,model and Chi-nese medicine groups(all P<0.05).There were no significant differences in live fetus count,dead fetus count,resorbed embryo count,implantation rate,embryo survival rate,pre-implantation loss rate,post-implantation loss rate,surviving embryo length,surviving placenta diameter,surviving embryo weight,or surviving embryo placenta weight among the blank,model,Chinese medicine groups and western medicine group Ⅰ(all P>0.05).No significant differences in uter-ine weight with embryos and uterine organ index with embryos were found between the model group and blank group(P>0.05),the uterine weight with embryos and uterine organ index with embryos in the western medicine group Ⅰ were signif-icantly lower than those in the blank group(both P<0.05).The serum levels of E2,P,and HCG in pregnant mice of the model group and western medicine group Ⅰ were significantly lower than those in the blank group and Chinese medicine group(all P<0.05).Conclusion BLAKF can significantly elevated the serum levels of E2,P,and HCG in pregnant DOR mice,thereby improving the endocrine environment for gestation.