和解生发汤对斑秃小鼠毛发再生及免疫细胞亚群的影响研究OA

[Objective]To investigate the effects of Hejie Shengfa Decoction(HJSFD)on hair regrowth and immune cell subsets in a mouse model of alopecia areata(AA),and to elucidate its potential immunoregulatory mechanisms.[Methods]An AA model was established in C3H/HeJ mice by topical application of imiquimod.The mice were randomly assigned to a blank control group,model group,low-,medium-,and high-dose HJSFD groups,and a compound glycyrrhizin tablet(CGT)group,with six mice in each group.All animals received intragastric administration for 14 consecutive days.Hair regrowth was evaluated by body weight monitoring,dermoscopy,H&E staining,and Ki67 immunofluorescence.Flow cytometry was used to detect peripheral CD8+T cells,Th1,Th17,Treg cells,and the Th17/Treg ratio.[Results]Mice in the model group exhibited typical AA manifestations,including epidermal thickening,reduced hair follicles,and lymphocytic infiltration.Compared with the model group,HJSFD promoted hair regeneration in a mouse model of alopecia areata,ameliorated pathological alterations in hair follicle structure,and enhanced Ki67 expression in dermal papilla cells,decreased CD8+T cells,Th1,and Th17 cells,elevated Treg cell levels,and restored the Th17/Treg balance.Among the treatment groups,the high-dose HJSFD group demonstrated the most pronounced therapeutic effects.[Conclusion]HJSFD promotes hair regrowth in AA mice,potentially by inhibiting the activation of CD8+,Th1,and Th17 cells,enhancing Treg-mediated immunoregulation,and restoring Th17/Treg homeostasis.These findings provide experimental evidence for the immunological mechanism of traditional Chinese medicine in the treatment of AA.