消痰散结方对结直肠癌细胞上皮间质转化和化疗耐药性的影响机制研究OA

Objective To investigate the effect of Xiaotan Sanjie Formula(消痰散结方,XTSJF)on epithelial-mesenchymal transition(EMT)and chemoresistance in colorectal cancer cells by regulating the focal adhesion kinase(FAK)/mitogen-activated extracellular signal-regulated kinase(MEK)/extracellular signal-regulated kinase(ERK)signaling pathway.Methods MTT assay was used to screen the optimal concentration of XTSJF.HCT116 cells were grouped into control group,L,M,H-XTSJF group(1.24、2.48、3.74mg/L)and XTSJF+ML-099 group(20 μmol/L MEK/ERK pathway activator ML-099+3.74 mg/L XTSJF);HCT116/5-fluorouracil cells were separated into HCT116/5-fluorouracil group and HCT116/5-fluorouracil+XTSJF group(3.74mg/L).The proliferation,migration,inva-sion,EMT,and pathway related proteins of HCT116 and HCT116/5-fluorouracil cells were detected.Results Compared with the control group,the scratch healing rate,number of invasive cells,FAK,MEK,ERK,Vimentin,and N-cadherin protein expression of HCT116 cells in the L,M,and H-XTSJF group were all reduced,the expression of E-cadherin protein increased(P<0.05),Compared with the H-XTSJF group,the above changes were partially reversed in the XTSJF+ML-099 group.Compared with the drug-resistant group,XTSJF treatment significantly reduced the IC50 value,proliferative activity,wound healing rate,number of invasive cells,and protein expression levels of FAK,MEK,ERK,Vimentin,and N-cadherin in HCT116/5-FU cells,while E-cadherin expression was signifi-cantly increased(P<0.05)..Conclusion XTSJF inhibits the proliferation,migration,invasion,and EMT of colorectal cancer cells by inhibiting the FAK/MEK/ERK pathway,thereby increasing the chemotherapy sensitivity of colorectal cancer cells to 5-fluorouracil.