营养元素添加对栀子根际土壤微生物多样性的影响OA

Rhizosphere microorganisms play a crucial role in plant growth and development,resistance to environmental stresses,and metabolic regulation.This study aims to clarify the effects of different concentrations of nitrogen(N),phosphorus(P),potassium(K),and magnesium(Mg)on rhizosphere soil microorganisms of Gardenia jasminoides seedlings.Four nutrient elements—N,P,K,and Mg—are selected,and the orthogonal experiment method is adopted.NH4NO3,KH2PO4,K2SO4 and MgSO4·7H2O are used to prepare different nutrient solutions with reference to Hoagland nutrient solution.Sampling is conducted 120 days after nutrient application to measure physiological indicators and active components.High-throughput sequencing technology is adopted to perform bioinformatics analysis on the microbial diversity and microbial community composition in its rhizosphere soil,respectively.The results show that based on the comprehensive ranking of principal component analysis(PCA),the optimal nutrient concentration combination for promoting the growth and physiology of gardenia seedlings is T8,consisting of 8 mmol/L NH4NO3,5 mmol/L KH2PO4,3 mmol/L K2SO4,8 mmol/L MgSO4·7H2O.The application of different nutrient elements reduces the abundance and diversity of the bacterial community in the rhizosphere soil of G.jasminoides,while increasing those of the fungal community.Among these treatments,T3 significantly enhances the abundance and diversity of the fungal community in the rhizosphere soil of G.jasminoides.Across all treatments,the dominant bacterial phyla in the rhizosphere soil of G.jasminoides are mainly Proteobacteria,Firmicutes,and Actinobacteria,and the dominant fungal phyla are scomycota,Basidiomycota,and Mucoromycota.Overall,the rational addition of nutrient elements can improve the composition and diversity of rhizosphere bacterial and fungal communities of G.jasminoides,providing new insights and theoretical support for the selection of fertilization strategies for G.jasminoides.