小麦黄花叶病田不同深度土壤致病力分析和病毒分离物鉴定OA

Wheat yellow mosaic virus(WYMV)is a significant soil-borne viral disease of wheat,and its incidence has shown an increasing trend in recent years.Field investigation reveals a severe occurrence of WYMV in Fei County,Linyi City,Shandong Province.Soil samples from different depths(0～10 cm,10～20 cm,20～30 cm,30～40 cm)in the affected area are collected and tested,confirming the presence of WYMV in the soil.Furthermore,the pathogenicity of the infested soil decreases progressively with increasing soil depth.The coat protein(CP)sequences of WYMV isolates from the diseased fields in Fei County are amplified and used to construct a phylogenetic tree.The results show that the CP sequences of WYMV isolates from Linyi cluster together and exhibit a close evolutionary relationship with WYMV isolates from Shaanxi(SX2 clone 22).The results of this study indicate that the topsoil in wheat yellow mosaic disease-infected fields possesses the strongest pathogenicity.Furthermore,more than two evolutionarily related WYMV isolates coexist in the same infected field.The phylogenetic analysis lays a foundation for further exploring the evolutionary direction of WYMV and designing drugs targeting conserved evolutionary sites.