基于双光子显微镜的光子嫩肤术后皮肤评估方法OA

This study enrolled 24 healthy adult female volunteers who underwent intense pulsed light(IPL)photo-rejuvenation treatment and were evaluated before and after the procedure using instrumental skin assessments.Among them,five participants underwent non-invasive in vivo imaging by two-photon microscopy.After IPL treatment,the skin barrier showed a transient disruption followed by progressive recovery and remodeling.Improvements in wrinkles at the outer canthus and nasolabial folds were observed from day 7 after treatment and persisted throughout the study period.Two-photon microscopy revealed enhanced stratum corneum organization and increased epidermal thickness.At the dermal-epidermal junction,greater structural undulation was observed,accompanied by increased collagen density and more organized elastic fiber architecture.Quantitative image analysis demonstrated an increase in the second harmonic generation-to-autofluorescence aging index of dermis(SAAID)and a decrease in the elastin-to-collagen optical ratio(ELCOR),indicating dermal remodeling and attenuation of photoaging-related characteristics.These findings suggest that two-photon microscopy enables objective,non-invasive,molecular-level assessment of IPL-induced barrier repair and dermal remodeling,providing mechanistic insights that complement conventional skin instrumental evaluations.