产期调节龙眼成花过程中顶芽和叶片的H2O2、NO含量分布变化OA
Effects of Off-season Longan on H2O2 and NO Content and Distribu-tion in Terminal Buds and Leaves during Flowering
氯酸钾是诱导龙眼非季节成花的关键药物,其强氧化性可引发树体氧化胁迫,而活性氧(如 H2O2)和活性氮(如 NO)作为氧化胁迫关键信号分子,其在顶芽及叶片中的分布与积累可能调控产期调节龙眼(反季节龙眼)花芽分化进程.本研究以9年生储良龙眼为试材,通过土施氯酸钾催花,结合外源喷施H2O2发生剂(MV)、H2O2清除剂(DMTU)、NO 发生剂(SNP)及 NO 合酶抑制剂(L-NNA),系统分析花芽分化过程中顶芽和叶片的 H2O2、NO 含量变化及组织分布特征.结果表明:MV 和 SNP 处理可显著提早产期调节龙眼花芽分化进程,催花 28 d 时纯花芽率(即完全花比例)显著高于对照;DMTU 和 L-NNA 处理则抑制或减缓成花过程.氯酸钾催花后 H2O2 在顶芽中主要分布于顶端分生组织及雏叶,叶片中先在主脉积累后扩散至叶肉,且叶片中 H2O2 峰值出现时间早于顶芽;SNP 和 MV 处理可增强 H2O2 荧光强度,DMTU 处理则显著清除 H2O2.NO 含量在催花 7 d 时达到峰值,SNP 处理可显著提高顶芽和叶片 NO 含量,L-NNA 处理对 NO 积累无显著影响,但抑制成花.综上,H2O2 和 NO 作为关键信号分子,共同促进产期调节龙眼成花,其含量变化与组织分布特征为解析氯酸钾诱导龙眼花芽分化的氧化胁迫机制提供理论依据.
Potassium chlorate is a key agent for inducing off-season flowering in longan(Dimocarpus longan Lour.),and its strong oxidizing property can cause oxidative stress in trees.Reactive oxygen species(e.g.,H2O2)and reactive ni-trogen species(e.g.,NO),as key signaling molecules of oxidative stress,and their distribution and accumulation in ter-minal buds and leaves may regulate the flower bud differentiation process of off-season longan.In this study,9-year-old'Chuliang'longan trees were used as materials.Potassium chlorate was applied to the soil for flower induction,com-bined with foliar spraying of H2O2 generator(methyl viologen,MV),H2O2 scavenger(dimethylthiourea,DMTU),NO generator(sodium nitroprusside,SNP)and NO synthase inhibitor(L-NNA).The content changes and tissue distribution characteristics of H2O2 and NO in terminal buds and leaves during flower bud differentiation were systematically ana-lyzed.The MV and SNP treatments significantly advanced the flower bud differentiation process of off-season longan,and the pure flower bud rate was significantly higher than that of the control at 28 days after treatment.DMTU and L-NNA treatments inhibited or slowed down the flowering process.H2O2 was mainly distributed in the apical meristem and rudimentary leaves of terminal buds,and first accumulated in the main veins of leaves and then diffused to the mesophyll.The peak of H2O2 in leaves appeared earlier than that in terminal buds.SNP and MV treatments could en-hance the fluorescence intensity of H2O2,while DMTU treatment significantly scavenged H2O2.The NO content reached a peak at 7 days after flower induction,SNP treatment could significantly increase the NO content in terminal buds and leaves,and L-NNA treatment had no significant effect on NO accumulation but inhibited flowering.In conclusion,H2O2 and NO,key signaling molecules,jointly promote the flowering of off-season longan.The content changes and tissue distribution characteristics would provide a theoretical basis for analyzing the oxidative stress mechanism of potassium chlorate-induced longan flower bud differentiation.
杨子琴;王家保;张蕾;洪继旺;李松刚
中国热带农业科学院热带作物品种资源研究所/国家热带果树品种改良中心,海南 海口 571101中国热带农业科学院热带作物品种资源研究所/国家热带果树品种改良中心,海南 海口 571101中国热带农业科学院热带作物品种资源研究所/国家热带果树品种改良中心,海南 海口 571101中国热带农业科学院热带作物品种资源研究所/国家热带果树品种改良中心,海南 海口 571101中国热带农业科学院热带作物品种资源研究所/国家热带果树品种改良中心,海南 海口 571101
农业科技
龙眼产期调节成花H2O2NO
longan(Dimocarpus longan Lour.)off-seasonfloweringH2O2NO
《热带作物学报》 2026 (8)
1990-1999,10
国家自然科学基金面上项目(No.32472693)海南省现代农业产业技术体系建设专项(No.HNARS-08).
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