一年生辣椒(Capsicum annuum)叶片白斑形成的遗传与组学特征OA

Peppers are an important vegetable and cash crop in China.Leaf color mutants serve as valuable materials for stud-ying plant photosynthesis,pigment metabolism,and stress response mechanisms.In this study,the albino mutant CA123(pa-ternal)of Capsicum annuum was crossed with the green-leaf inbred line CaY6(maternal)to generate an F2 segregating popu-lation.Using integrated multi-omics approaches,including bulked segregant RNA-Seq(BSR-Seq),transcriptome(RNA-Seq)and metabolomics analysis,we elucidated the genetic mechanism of leaf albinism in peppers.BSR-Seq analysis localized key genes controlling the albino trait to two regions on pepper chromosome 12.Within the two loci,90 functional mutations were detected,and five candidate genes were identified:ZLC12G0008580(CLC-A),ZLC12G0018770(CYP71AU50),ZLC12G0017660(PCMP-H81),ZLC12G0009880(PCMP-E26),and ZLC12G0018520(FBN5).The comparative transcriptome analysis identified numerous differentially expressed genes(DEGs).KEGG enrichment analysis indicated that these genes were significantly enriched in diverse pathways,including MAPK signaling-plant,glutathione metabolism,pho-tosynthesis-antenna proteins,Calvin cycle,and brassinosteroid biosynthesis.Significant expression changes were observed in multiple key genes,including receptor kinases(XA21),transcription factors(WRKY24),and enzymes such as peroxidases and glutathione S-transferases.Metabolomics analysis identified hundreds of differentially expressed metabolites,primarily en-riched in pathways such as glyoxylate and dicarboxylic acid metabolism,alkaloid biosynthesis,and ABC transporters.Metab-olites such as citric acid,linolenic acid,jasmonic acid,and malic acid exhibited significantly altered levels in albino leaves.WGCNA analysis revealed that the antique white 4 module was significantly correlated with the albino phenotype,from which multiple hub genes,including transcription factors PBF1,HY5,MOF1,and BBX22,were identified.In summary,this study analyzed the characteristics associated with leaf bleaching in peppers from three perspectives:genetic mapping,transcriptional regulation,and metabolomics.It provides an important reference for understanding the patterns of leaf color variation in pep-pers and lays the foundation for the further development of molecular markers for leaf color in peppers to aid in breeding.