木薯转录因子MeMYBS1的克隆与表达分析OA

MYB transcription factors play crucial roles in plant growth and development,secondary metabolism,and responses to environmental stresses.In this study,a 1R-MYB transcription factor gene,MeMYBS1,was cloned from cassava(Manihot esculenta Crantz)cultivar South China 8(SC8)using RT-PCR technology.Bioinformatics analysis using online tools such as ExPasy and NCBI-CDD revealed that the full-length of MeMYBS1 was 1008 bp,encoding a protein of 335 amino acids with a predicted molecular weight of 36.3 kDa and an isoelectric point of 6.46.Subcellular localization analysis via PEG-mediated transient expression in cassava protoplasts demonstrated that MeMYBS1 was localized in the nucleus.qRT-PCR tissue expression analysis showed that MeMYBS1 exhibited high expression abun-dance in cassava storage roots.Furthermore,during the process of post-harvest physiological deterioration(PPD)in South China 9(SC9)storage roots,the expression level of MeMYBS1 showed a significant downward trend as storage time extended.The results preliminarily characterized the expression patterns of MeMYBS1 during cassava PPD,pro-viding a theoretical foundation for further elucidating the molecular mechanisms by which this gene regulates PPD in cassava.