1株港口码头石油降解菌的分离与鉴定OA

Oil pollution has a profound effect on the ecology along the port.It is an important direction for oil pollution remediation to study and excavate microorganisms capable of oil degradation.Through enrichment culture,oil plate screening and blood plate identification,a strain of oil-degrading bacteria producing surfactant was screened from the dock coast.Based on 16S rRNA gene sequencing analysis,the bacterium was identified as Microbacterium esteraromaticum and named M.esteraromaticum BS-1.The fermentation solution of the strain BS-1 could reduce the surface tension of water from 57.1 mN/m to 28.7 mN/m,and the degradation rate of crude oil reached 31.6%.The surfactants produced by BS-1 growth and metabolism were identified as glycolipids containing aldose groups by TLC thin layer chromatography,characteristic color development and infrared spectroscopy.The results can provide reference for developing new biosurfactants and further studying oil pollution bioremediation technology.