木质纤维素降解菌的筛选、鉴定及酶学性质OA
Screening and Identification of Lignocellulose Degrading Bacteria and Study in Its Enzymatic Properties
筛选高效分解木质纤维素菌株并研究其酶学性质,为木质纤维素的高值利用提供试验数据和理论指导.本研究从茂名露天矿生态公园筛选到一株高地芽孢杆菌(B.altitudinis),其分泌的木聚糖酶和内切葡聚糖酶的最适催化温度分别为50,60℃,pH 分别为 7、6,并且 2 种酶在低于 40℃时有良好的热稳定性,而木聚糖酶在中碱性条件下能保持很好的 pH 稳定性.金属离子和化学试剂对木聚糖酶有不同程度的抑制作用,而 Cu2+和 SDS 对内切葡聚糖酶有轻微的促进作用.B.altitudinis strain OU9 所产木质纤维素降解酶的优异性能为木质纤维素的高效绿色降解和开发应用提供巨大的潜在价值.
To provide experimental data and theoretical guidance for the high-value utilization of lignocellulose,strains with high decomposition efficiency for lignocellulose were screened and their enzymatic properties were studied.In this study,a strain of Bacillus subtilis was screened from Guangdong Maoming Lutiankuang Ecology Park.The xylanase and endoglucanase produced by the strain had optimum temperatures of 50℃and 60℃,and optimum pH of 7.0 and 6.0,respectively.while xylanase maintained good pH stability under medium-alkaline conditions.Metal ions and chemical reagents have varying degrees of inhibitory effects on xylanase,while Cu2+and SDS have a slight promoting effect on endoglucanase.The excellent performance of lignocellulose degrading enzymes secreted by Bacillus altitudinis strain OU9 provides enormous potential value for the efficient green degradation and exploitation as well as application of lignocellulose.
区晓阳;廖育祥;李莉梅;陆光远
广东石油化工学院 生物与食品工程学院,广东 茂名 525000||广东省农产品绿色与智能生产重点实验室,广东 茂名 525000广东石油化工学院 生物与食品工程学院,广东 茂名 525000广东石油化工学院 生物与食品工程学院,广东 茂名 525000广东石油化工学院 生物与食品工程学院,广东 茂名 525000||广东省农产品绿色与智能生产重点实验室,广东 茂名 525000
资源环境
木质纤维素菌株筛选木聚糖酶内切葡聚糖酶酶学性质
lignocellulosestrain screeningxylanaseendoglucanaseenzymatic properties
《广东石油化工学院学报》 2026 (3)
26-31,6
广东石油化工学院人才引进项目(2020rc046)广东省乡村振兴科技专项(2022DZXHT072,2023S018087,2023S002021)
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