洗涤红细胞蛋白组学和代谢组学分析及关键质量评价的研究OA

Objective This study aimed to determine the quality indicators,proteomic and metabolomic profiles of washed red blood cells(WRBCs),and further evaluate their quality alterations at the end of storage via correlation analysis.Methods Suspended RBCs were categorized into two groups:the control group(Control5w),which did not undergo washing treatment,and the saline-washed group(Saline5w).Following storage at 4℃,samples were collected at the end of storage,and relevant quality control indicators for suspended RBCs were examined.Proteomic and metabolomic analyses were performed to assess changes in biological functions and correlations during the storage process of the two groups using bioinformatics analysis.Results After 35 days of storage at 4℃in the saline group,all RBC indicators complied with national standards for the quality of WRBCs.The comprehensive integration and analysis of proteomics and metabolomics data have elucidated alterations in the protein and metabolite composition of RBCs washed with saline.This was achieved by examining the correlations among various biomolecular levels.Conclusion This study substantiates the investigation of the physiological and biochemical functions of washed RBCs through the application of advanced proteomic and metabolomic methodologies.