毛竹叶总黄酮的超声波辅助酶法提取及其成分分析OA

Moso bamboo(Phyllostachys edulis(Carrière)J.Houz)leaves were used as raw material to extract flavonoids via ultrasound-assisted enzymatic method.Using 60%ethanol as the extraction solvent,the process conditions were optimized by response surface method on the basis of single-factor test.The results showed that the optimal extraction conditions for 1 g P.edulis leaves powder were as follows:cellulose addition of 1.4%(based on the mass of bamboo leaves),liquid-solid ratio of 34∶1(mL∶g),extraction temperature of 55℃,extraction time of 40 min,and ultrasound power of 336 W.Under these conditions,the total flavonoid yield from bamboo leaves reached 2.460%.The P.edulis leaves before and after extraction were characterized by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR)and nanoparticle size analyzer.Analysis results revealed that ultrasound-assisted enzymatic treatment resulted in a rougher surface of the enzymatically treated material and disrupted cell wall structures,facilitating the transfer of flavonoids from the cells into the extraction solution and thereby enhancing extraction efficiency.By comparing four methods of ethanol treatment,ultrasound treatment,enzymatic hydrolysis,and ultrasound enzymatic hydrolysis,it was found that the yield of total flavonoid,the yield of total polyphenol and antioxidant activity of bamboo leaves flavonoid extracted by ultrasound-assisted enzymatic hydrolysis were 2.465%,10.874%and 87.791%,respectively,which were the highest.High performance liquid chromatography(HPLC)analysis revealed that the contents of various flavonoids(vitexin,isovitexin,orientin,isoorientin and apigenin)in bamboo leaves extract treated by ultrasound-assisted enzymatic method were significantly higher than those in other treatment groups.