抗帕颗粒联合益生菌对帕金森病模型小鼠血清短链脂肪酸的协同调节作用OA

Objective To investigate the effects of single use of Chinese herbal formula Kangpa Granule and probiotics,and their combination on serum short-chain fatty acid(SCFAs)metabolism in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced mouse model of Parkinson's disease(PD).Methods A total of 100 male C57BL/6 mice were randomly divided into normal control group(group A),PD model group(group B),PD probiotic intervention group(group C1),PD Kangpa Granule intervention group(group C2),and PD probiotic and Kangpa Granule combined intervention group(group C3).Except for group A,all groups received intraperitoneal injection of MPTP 40 mg/(kg·d)for 5 consecutive days to establish the PD model.After modeling,each group received the corresponding intragastric intervention for 12 weeks.Serum samples were collected before the post-modeling intervention and after the 12-week intervention.Gas chromatography-mass spectrometry(GC-MS)was used to measure the levels of eight SCFAs(acetic acid,propionic acid,butyric acid,isobutyric acid,valeric acid,isovaleric acid,caproic acid,and isocaproic acid),and principal component analysis(PC A)was performed.Results Compared with group A,the serum levels of acetic acid,butyric acid,and caproic acid in group B were decreased both before the post-modeling intervention and after the 12-week intervention,and the differences were statistically significant(P＜0.01,remaining significant difference after Bonferroni correction).After 12-week interven-tion,the serum levels of acetic acid,butyric acid,and caproic acid in groups C1,C2,and C3 were significantly higher than those before the intervention(P＜0.05 or P＜0.01),mostly approaching to those of group A.After 12-week intervention,the serum levels of propionic acid and valeric acid in group C3 were significantly higher than those in groups B,A,C1,and C2(P＜0.01,remaining significant difference after Bonferroni correction).There were no statistically significant differences in the serum levels of isobutyric acid,isovaleric acid,and isocaproic acid among the groups at any time point(P＞0.05).PCA results showed that the overall SCFA metabolic profile of group C3 was clearly separated from those of the other groups after the intervention,forming a distinct metabolic homeostasis.Conclusion MPTP-induced PD mice exhibit SCFA metabolic dysregulation character-ized by decreased serum levels of acetic acid,butyric acid,and caproic acid.Single use of probiot-ics and Kangpa Granule can partially correct this dysregulation,whereas their combined use syner-gistically elevates the levels of propionic acid and valeric acid,effectively remodels the SCFA meta-bolic profile,and provides a preliminary experimental basis for gut-brain axis-based therapeutic strategies for PD.