脂肪干细胞对急性胰腺炎犬线粒体生物合成的影响OA

To investigate the therapeutic efficacy of adipose-derived stem cells(ADSCs)against acute pancreatitis(AP)in canines,nine native Chinese rural canines were randomly divided into the control(CON)group,AP group,and ADSCs group.An incision was performed on the intestinal wall opposite the mesentery of the major duodenal papilla.The AP and ADSCs groups were subjected to retrograde pancreaticobiliary infusion of 5%sodium taurocholate(0.1 mL·kg-1)and trypsin(3 500 U·kg-1),while the CON group received an identical volume of sterile normal saline.Postoperatively,canines in the ADSCs group were intravenously administrated ADSCs at 1×106 cells·kg-1 for intervention,and the CON and AP groups were injected with equal-volume normal saline.Blood and pancreatic tissue samples were collected at 24 h post-operation to detect clinical manifestations,pain scores,pepsin and trypsin activities,ATP content,Ca-ATPase activity and the expression of mitochondrial biogenesis markers.The results showed that physiological indicators of all experimental canines remained stable intraoperatively and postoperatively.In the AP group,the activities of pepsin and typsin were significantly increased,while Ca-ATPase,ATP concentration and the expression levels of mitochondrial biogenesis markers were markedly decreased.ADSCs treatment significantly reduced elevated pepsin and trypsin activities and upregulated the expression of mitochondrial biogenesis markers.In conclusion,the surgically established canine acute pancreatitis model has reliable safety.ADSCs protect pancreatic tissues via restraining pepsin and trypsin secretion and enhancing mitochondrial biogenesis,which provides experimental data for exploring AP pathogenesis and clinical application of ADSCs.