绿豆VrWRKY50基因克隆及在盐碱胁迫下的功能研究OA

Salt-alkaline stress is one of the major factors limiting mung bean production.To investigate the function of VrWRKY50 under saline-alkaline stress,transgenic mung bean hairy roots with VrWRKY50 overexpression(OE)and RNA interference(RNAi)were constructed.The materials were treated with mixed saline-alkaline solution(NaCl∶Na2SO4∶NaHCO3∶Na2CO3=1∶9∶9∶1,pH 8.5)for 3 days,with distilled water set as the control.Plant growth and physiological indices were subsequently determined.The results showed that VrWRKY50 was mainly localized in the cell nucleus.Transgenic yeast exhibited better growth performance than the empty control under saline-alkaline conditions.Compared with WT and RNAi roots,VrWRKY50-OE hairy roots presented vigorous root growth,accompanied by significantly increased contents of osmotic adjustment substances and enhanced activities of antioxidant enzymes,while the accumulation of reactive oxygen species and malondialdehyde content decreased markedly.These findings indicate that VrWRKY50 acts as positive regulator in mung bean response to saline-alkaline stress and can improve the saline-alkaline tolerance of plants.