水葫芦与牛粪堆制的配比优化及其对蚯蚓肠道菌群的影响OA

To promote the resource utilization of water hyacinth and cattle manure,five treatment groups with water hyacinth mass percentages of 50%,67%,75%,80%,and 100%were set up as vermicomposting substrates with different ratios of cattle manure to water hyacinth.It was shown by the results that the moisture content,total organic carbon,total nitrogen content and C/N ratio of the substrates could be effectively regulated by the addition of water hyacinth,and the feeding rate of earthworms was enhanced to some extent.However,the growth of earthworm body weight and body length was significantly inhibited by a high water hyacinth mass percentage(≥80%).Through intestinal dissection and high-throughput sequencing analysis of gut microbiota,it was found that the diversity and richness of the gut microbial community were significantly reduced by the high water hyacinth percentage treatment group,and the community structure was altered.Specifically,the abundance of Proteobacteria was increased,while that of Planctomycetes was decreased,and this change could affect the digestive and absorptive functions and overall gut health of earthworms.From the perspective of healthy breeding and conversion efficiency,the optimal ratio of water hyacinth was recommended as 75%by the study,by which efficient conversion of water hyacinth could be achieved and the adverse effects on the normal growth of earthworms were minimized to the greatest extent.