不同作物轮作下的植烟土壤微生物群落结构和生态功能OA

[Objective]The effects of different crop rotations on the microbial community structure and ecological function of tobacco-growing soil were investigated,which provided theoretical basis for the green prevention and control strategy of tobacco soil-borne diseases based on microbial regulation.[Method]Illumina NovaSeq high-throughput sequencing technology was used to analyze the composition,network structure and microbial function of soil fungal and bacterial communities in the rotation of Longya cabbage,Vicia villosa and pea with tobacco.[Result]Microbial community structure analysis showed that compared with winter fallow,all three types of subsequent crops reduced the α-diversity of soil fungal and bacterial communities,and there were significant differences in β-diversity as well;The relative abundance of Mortierella,Penicillium and Fusarium were increased,while the relative abundance of Plectosphaerella was decreased.The relative abundance of Proteobacteria was increased as that of Acidobacteriota was decreased;Mortierella and Penicillium were mainly involved in the establishment of fungal correlation network in soil,while Chujaibacter and Acidothermus were mainly involved in the establishment of bacterial correlation network.Ecological function analysis showed that the three types of aftercrop could significantly increase the abundance of soil saprotrophic fungi and significantly reduce the abundance of pathotrophic fungi,but there was no significant difference in the function and phenotype of bacterial communities.[Conclusion]The three kinds of subsequent crops,including Longya cabbage,V.villosa and pea,can reduce the microbial diversity and the relative abundance of pathotrophic fungi in tobacco-growing soil,and can increase the relative abundance of beneficial microorganisms.