基于重测序定位特色稻贵州禾RIL群体粒型性状QTLOA

[Objective]The QTL loci related to grain traits of Guizhouhe were probed by constructing rice recombinant inbred lines population to provide a theoretical basis for analyzing the grain formation mechanism of Guizhouhe and rice breeding.[Method]The QTLs related to grain traits of Guizhouhe were detected by using a high-density genetic map built from the rice recombinant inbred line population of Goudang 3(special rice Guizhouhe)×Huazhan(a quality restorer)based on the two-year's phenotype data.[Result]A total of seven QTLs in RIL population were detected and located on chromosome 1,2 and 5,and their LOD value and contribution rate were 3.29-15.98 and 7.67%-37.03%,respectively.Three QTLs loci related to grain width trait were qGW-2,qGW-5-1 and qGW-5-2.Four QTLs loci related to thousand-grain weight trait were qTGW-1-1,qTGW-5-1,qTGW-1-2 and qTGW-5-2.Other 5 QTLs might be new loci including qGW-2(related to grain width)and qTGW-1-1,qTGW-5-1,qTGW-1-2 and qTGW-5-2(related to thousand-grain weight).The qGW-2 was detected for two consecutive years.[Conclusion]Three QTLs(qGW-2,qGW-5-1 and qGW-5-2)related to grain width,and four QTLs(qTGW-1-1,qTGW-5-1,qTGW-1-2 and qTGW-5-2)related to thousand-grain weight are detected from the RIL population of Goudang 3(special rice Guizhouhe)×Huazhan(a quality restorer).The qGW-5-1 gene is involved in regulation of grain length,grain width and grain thickness and the qGW-5-2 is involved in regulation of grain width.The qGW-2,qTGW-1-1,qTGW-5-1,qTGW-1-2 and qTGW-5-2 are five new QTLs.