喷施谷氨酸对苦荞麦幼苗生长及相关基因表达的影响OA

[Objective]The effects of glutamic acid on growth and related gene expression of tartary buckwheat seedlings were explored to provide a theoretical basis for increasing crop biomass by using exogenous substances.[Method]The related growth indexes of tartary buckwheat seedlings of Chuanqiao No.1 sprayed with different concentrations(10 μmol/L,20 μmol/L,30 μmol/L,40 μmol/L,50 μmol/L and 60 μmol/L)of glutamic acid were determined.The expression level of the genes related to chlorophyll synthesis and N transport was analyzed to investigate the effects of spraying glutamic acid on growth of tartary buckwheat seedlings.[Result]The height,fresh weight,SPAD value and N content of tartary buckwheat seedlings sprayed with 40 µmol/L glutamic acid increased by 8%,28%,52%and 35%compared with CK(spraying clean water)respectively,which indicated that spraying glutamic acid promoted growth of tartary buckwheat seedlings.Spraying 40 µmol/L glutamic acid obviously promoted expression of FtDCL and FtGLK1 genes related to chlorophyll synthesis and FtAMT1-1 and FtNRT2.3 genes related to N transport in tartary buckwheat.The maximum expression level of FtDCL and FtGLK1 genes at 12 h increased by 7.63 times and 8.01 times compared with CK,respectively.The maximum expression level of FtAMT1-1 and FtNRT2.3 genes at 12 h and 24 h increased by 8.33 times and 6.21 times compared with CK,respectively.[Conclusion]Spraying 40 µmol/L glutamic acid not only increases height,fresh weight,and chlorophyll and N content of tartary buckwheat seedlings but also promotes expression of genes related to chlorophyll synthesis and N transport of tartary buckwheat seedlings.