薏苡ClAP2/ERF基因家族全基因组鉴定及表达模式分析OA

[Objective]The structure and function of ClAP2/ERF gene family members in coix(Coix lacryma-jobi)were investigated to provide a reference for analyzing the biological function of ClAP2/ERF gene family.[Method]The members of ClAP2/ERF gene family in coix were identified by bioinformatics based on the genome-wide data of coix,the physicochemical properties,phylogenetic evolution,chromosomal localization,gene structure,conserved motif identification,promoter cis-acting elements and inter-species collinearity of the members were analyzed,and the expression pattern of ClAP2/ERF gene family was analyzed,also.[Result]A total of 165 ClAP2/ERF genes were identified from the genome of Coix lacryma-jobi,encoding 128-711 amino acids with a relative molecular mass ranging from 13.31 to 74.33 kDa and an isoelectric point ranging from 4.3 to 10.8.The coix AP2/ERF proteins were classified into 5 subfamilies of AP2,ERF,DREB,RAV and Soloist.Among them,the proportion of DREB subfamily and Soloist subfamily was the highest and the lowest,respectively.The members of ClAP2/ERF gene family were unevenly distributed on 10 chromosomes.The gene structure of coix AP2 subfamily was the most complex,containing 5-10 exons and 4-9 introns.The DREB and ERF subfamilies contained 1-3 introns.The Soloist and RAV subfamilies contained an exon only.Coix had 17 480 pairs of genes and 29 048 pairs of genes in common association with rice and sorghum,respectively.The promoter region of ClAP2/ERF gene contained 14 types of cis-acting elements,and the number of light-responsive elements was the highest,followed by the environmental stress elements.The members of ClAP2/ERF gene family in coix showed differential expression in different tissues.75,67 and 68 ClAP2/ERF genes were detected in leaves,roots,and stems,respectively.Among them,45 ClAP2/ERF genes were simultaneously expressed in roots,stems,and leaves.ClAP2/ERF13,ClAP2/ERF22,ClAP2/ERF26,ClAP2/ERF49,ClAP2/ERF78,ClAP2/ERF80,and ClAP2/ERF115 genes all were highly expressed in roots,stems and leaves simultaneously.[Conclusion]The ClAP2/ERF protein can be classified into 5 subfamilies.The members of ClAP2/ERF gene family have the colinearity with rice and sorghum,and their evolution is relatively conservative.The promoter contains cis-acting elements for light response and stress response.The expression of the cis-acting elements varies in different tissues.It is speculated that ClAP2/ERF gene family is involved in growth,development and adversity regulation process of coix.