毛乌素沙地林药间作对根际土壤微生物群落结构的影响OA
Effects of forest-medicinal plant intercropping on rhizosphere soil microbial community structure in the Mu Us Sandy Land
在毛乌素沙地推广林药间作模式,是实现生态与经济效益协同提升的重要途径.于2021年在位于毛乌素沙地西南缘的灵武大泉林场开展田间试验,设置长柄扁桃分别与银柴胡(PR)、金银花(PL)及黄芩(PS)的间作处理,并以长柄扁桃单作(CK)为对照,2023年系统测定不同处理的pH值及土壤养分,并利用高通量测序技术分析根际土壤微生物的群落结构.结果表明:(1)间作模式显著影响pH值及土壤养分,其中PR处理的全氮(333.67 mg·kg-1)和有机质(5.94 g·kg-1)含量分别较CK提高80.9%和75.7%,且pH值最低(8.57);(2)PR处理的根际土壤细菌OTU数目最高为3435,真菌ACE指数(656.31)、Chao1指数(654.14)显著高于其他处理(P<0.05);(3)在微生物群落组成方面,PR模式提高了放线菌门、绿弯菌门和被孢霉菌门等有益菌的相对丰度,同时降低了镰刀菌属等病原菌的比例;(4)Pearson相关性和冗余分析表明,全氮、有机质、速效钾是影响真菌群落的关键因子,而有效磷和pH值对细菌群落的结构和多样性产生影响.综上,长柄扁桃与银柴胡间作(PR)能显著提升土壤养分、优化微生物群落结构,是一种适宜在毛乌素沙地推广的林药复合种植模式.
The popularization of forest-medicinal herb intercropping in the Mu Us Sandy Land serves as a vital means to synergistically enhance ecological and economic benefits.In this study,a field experiment was conduct-ed in 2021 at Daquan Forest Farm(Lingwu,China)located on the southwestern edge of the Mu Us Sandy Land.Three intercropping treatments were established,namely,Amygdalus pedunculata intercropped with Stellaria di-chotoma var.lanceolata(PR),Lonicera japonica,and Scutellaria baicalensis,with monocropped Amygdalus pe-dunculata set as the control.In 2023,the soil pH and nutrient contents under different treatments were systemati-cally determined,and high-throughput sequencing technology was used to analyze the community structure of rhi-zosphere soil microorganisms.Firstly,intercropping patterns exerted a significant effect on soil pH and nutrient contents.Specifically,the contents of total nitrogen(333.67 mg·kg-1)and soil organic matter(5.94 g·kg-1)under PR treatment was increased by 80.9%and 75.7%,respectively,compared with the PP treatment,with the lowest pH value(8.57).Secondly,the number of bacterial operational taxonomic units in the rhizosphere soil under PR treatment was the highest(3435),and its fungal Abundance-based Coverage Estimator index(656.31)and Chao1 index(654.14)were significantly higher than those recorded under other treatments(P<0.05).Thirdly,regarding microbial community composition,the PR pattern increased the relative abundance of beneficial taxa,including Actinobacteriota,Chloroflexi,and Mortierellomycota,while reducing the proportion of pathogenic genera such as Fusarium.Fourthly,Pearson correlation and redundancy analysis revealed that total nitrogen,organic matter,and available potassium were key factors affecting the fungal community,while available phosphorus and pH influ-enced the structure and diversity of the bacterial community.In conclusion,PR treatment may significantly im-prove soil chemical properties and optimize the microbial community structure;hence,it may be a suitable forest-medicinal intercropping pattern for popularization in the Mu Us Sandy Land.
苟莉;王瑞霞;包玉国;朱林
宁夏大学林业与草业学院,宁夏 银川 750021||西北退化生态系统恢复与重建教育部重点实验室,宁夏大学西北土地退化与生态系统恢复国家重点实验室培养基地,宁夏 银川 750021||宁夏白芨滩森林生态系统定位观测研究站,宁夏 灵武 750499宁夏灵武白芨滩国家级自然保护区管理区,宁夏 灵武 750499固原市原州区蝉塔山林场,宁夏 固原 756000西北退化生态系统恢复与重建教育部重点实验室,宁夏大学西北土地退化与生态系统恢复国家重点实验室培养基地,宁夏 银川 750021||宁夏白芨滩森林生态系统定位观测研究站,宁夏 灵武 750499||宁夏大学生态环境学院,宁夏 银川 750021
毛乌素沙地长柄扁桃林药间作土壤养分微生物群落
Mu Us Sandy LandAmygdalus pedunculataforest-medicinal plant intercroppingsoil nutrientsmicrobial community
《干旱区研究》 2026 (8)
1644-1654,11
宁夏回族自治区重点研发计划项目(2022BEG02012,2026BEG01003)
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