八宝丹对5-氟尿嘧啶所致大鼠心脏损伤的保护作用OA

Objective:This study aims to investigate the cardioprotective effect of Babao Dan(BBD)against 5-fluorouracil(5-FU)-induced cardiac injury in rats,and to explore the underlying mechanism.Methods:Thirty male Wistar rats were randomly divided into three groups:the control group,the model group,and the treatment group,with 10 rats in each group.Rats in the control group received an intraperitoneal(i.p.)injection of normal saline;rats in the model group received an i.p.injection of 5-FU for 3 consecutive weeks;rats in the treatment group received 5-FU injection combined with concurrent intragastric administration of BBD for 3 consecutive weeks.Cardiac function was evaluated via echocardiography to assess ejection fraction(EF)and left ventricular fractional shortening(LVFS),in combination with spectrophotometric colorimetric assays to detect levels of creatine kinase(CK),creatine kinase-MB isoenzyme(CK-MB),and lactate dehydrogenase(LDH).Histopathological changes in cardiac tissue were assessed via hematoxylin-eosin(HE)staining.Myocardial apoptosis was detected via terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)staining.Car-diomyocyte mitochondrial damage was evaluated via transmission electron microscopy(TEM)and MitoTracker Deep Red FM staining.Results:Compared with the control group,the model group exhibited a significant decrease in EF and LVFS(P<0.05),along with re-duced levels of CK and CK-MB(P<0.05).Compared with the model group,the treatment group showed significantly increased EF and LVFS(P<0.05)and decreased LDH levels(P<0.05).HE staining revealed severe myocardial injury in the model group,characterized by widened interstitial spaces and disorganized cardiomyocyte arrangement,while these pathological changes were alleviated in the treat-ment group.TUNEL staining demonstrated that the number of apoptotic cardiomyocytes was significantly higher in the model group than in the control group(P<0.05),and this elevation was significantly attenuated in the treatment group(P<0.05).TEM combined with MitoTracker Deep Red staining showed that mitochondrial count and fluorescence intensity were significantly lower in the model group than in the control group(P<0.05);compared with the model group,the treatment group had an increased mitochondrial count,but the change in fluorescence intensity did not reach statistical significance(P>0.05).Conclusion:5-Fluorouracil induces significant cardio-toxicity in rats,which is characterized by impaired cardiac function,myocardial injury,and reduced myocardial mitochondrial content.Babao Dan can attenuate 5-FU-induced cardiotoxicity and exert cardioprotective effects.